mouse anti cycb f2f4 Search Results


anti cycb f2f4  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank anti cycb f2f4
Anti Cycb F2f4, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cycb f2f4/product/Developmental Studies Hybridoma Bank
Average 94 stars, based on 1 article reviews
anti cycb f2f4 - by Bioz Stars, 2026-03
94/100 stars
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anti-cycb  (Becton Dickinson)
90
Becton Dickinson anti-cycb
Sequences in the <t>cycB</t> 3′ UTR are required for translational repression. (A) 3′ RACE PCR on the cycB transcript from RNA collected from wild-type testis, wild-type ovary, bam mutant testis (spermatogonia accumulate, and spermatocytes and spermatids are absent). Forward primer for 3′ RACE was nearly identical to primer #1 in C, just 4 bases longer. (B) RT-PCR from RNA collected from wild-type testis, wild-type ovary, bam mutant testis and rbp4 mutant testis, using primers #1-3, shown in C. Predicted products: 124 bp from short and long form; 227 bp from long form only. (C) Schematic of the cycB 3′ UTR as detected in ovary and spermatogonia versus in spermatocytes. NRE, Nanos response element. (D) A conserved proportion of the short cycB 3′ UTR, with mut9+5 variation created by site-directed mutagenesis (mutated nucleotides shown in lowercase). (E) Schematic of CycB-eYFP in vivo reporter. <t>(F-J)</t> <t>Anti-GFP</t> immunofluorescence on: (F) CycB-eYFP in wild-type testis, (G) CycB-eYFP in rbp4 mutant testis and (H) CycB-eYFP-mut9+5 in wild-type testis. White lines indicate early spermatocytes. Scale bar: 100 µm in H. (I,J) RT-PCR on CycB-eYFP, CycB-eYFP in rbp4 and CycB-eYFP-mut9+5 reporters. (I) Amplifying eYFP (313-bp expected product) and (control) GAPDH2 (100 bp) to assay reporter transcript levels. (J) Using an eYFP forward primer and cycB 3′ UTR reverse primers 2 and 3. Predicted products: 157 bp from short and long form; 260 bp from long form only – not detected. (K) Anti-GFP western blot of a biotin RNA pull-down from Rbp4-eYFP or Ubi-GFP testis extract. Wild-type and mutant biotin probes as indicated. (L) Quantification via ImageJ of three independent biotin RNA pull-downs from Rbp4-eYFP. The mean of the 10% input bands was set to 1; a value of 2 for the wild-type probe indicates that that probe pulled down ∼20% of the Rbp4-eYFP input. Error bars indicate s.e.m.
Anti Cycb, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-cycb/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
anti-cycb - by Bioz Stars, 2026-03
90/100 stars
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cycb  (Developmental Studies Hybridoma Bank)
90
Developmental Studies Hybridoma Bank cycb
Sequences in the <t>cycB</t> 3′ UTR are required for translational repression. (A) 3′ RACE PCR on the cycB transcript from RNA collected from wild-type testis, wild-type ovary, bam mutant testis (spermatogonia accumulate, and spermatocytes and spermatids are absent). Forward primer for 3′ RACE was nearly identical to primer #1 in C, just 4 bases longer. (B) RT-PCR from RNA collected from wild-type testis, wild-type ovary, bam mutant testis and rbp4 mutant testis, using primers #1-3, shown in C. Predicted products: 124 bp from short and long form; 227 bp from long form only. (C) Schematic of the cycB 3′ UTR as detected in ovary and spermatogonia versus in spermatocytes. NRE, Nanos response element. (D) A conserved proportion of the short cycB 3′ UTR, with mut9+5 variation created by site-directed mutagenesis (mutated nucleotides shown in lowercase). (E) Schematic of CycB-eYFP in vivo reporter. <t>(F-J)</t> <t>Anti-GFP</t> immunofluorescence on: (F) CycB-eYFP in wild-type testis, (G) CycB-eYFP in rbp4 mutant testis and (H) CycB-eYFP-mut9+5 in wild-type testis. White lines indicate early spermatocytes. Scale bar: 100 µm in H. (I,J) RT-PCR on CycB-eYFP, CycB-eYFP in rbp4 and CycB-eYFP-mut9+5 reporters. (I) Amplifying eYFP (313-bp expected product) and (control) GAPDH2 (100 bp) to assay reporter transcript levels. (J) Using an eYFP forward primer and cycB 3′ UTR reverse primers 2 and 3. Predicted products: 157 bp from short and long form; 260 bp from long form only – not detected. (K) Anti-GFP western blot of a biotin RNA pull-down from Rbp4-eYFP or Ubi-GFP testis extract. Wild-type and mutant biotin probes as indicated. (L) Quantification via ImageJ of three independent biotin RNA pull-downs from Rbp4-eYFP. The mean of the 10% input bands was set to 1; a value of 2 for the wild-type probe indicates that that probe pulled down ∼20% of the Rbp4-eYFP input. Error bars indicate s.e.m.
Cycb, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cycb/product/Developmental Studies Hybridoma Bank
Average 90 stars, based on 1 article reviews
cycb - by Bioz Stars, 2026-03
90/100 stars
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mouse anti cycb  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank mouse anti cycb
Sequences in the <t>cycB</t> 3′ UTR are required for translational repression. (A) 3′ RACE PCR on the cycB transcript from RNA collected from wild-type testis, wild-type ovary, bam mutant testis (spermatogonia accumulate, and spermatocytes and spermatids are absent). Forward primer for 3′ RACE was nearly identical to primer #1 in C, just 4 bases longer. (B) RT-PCR from RNA collected from wild-type testis, wild-type ovary, bam mutant testis and rbp4 mutant testis, using primers #1-3, shown in C. Predicted products: 124 bp from short and long form; 227 bp from long form only. (C) Schematic of the cycB 3′ UTR as detected in ovary and spermatogonia versus in spermatocytes. NRE, Nanos response element. (D) A conserved proportion of the short cycB 3′ UTR, with mut9+5 variation created by site-directed mutagenesis (mutated nucleotides shown in lowercase). (E) Schematic of CycB-eYFP in vivo reporter. <t>(F-J)</t> <t>Anti-GFP</t> immunofluorescence on: (F) CycB-eYFP in wild-type testis, (G) CycB-eYFP in rbp4 mutant testis and (H) CycB-eYFP-mut9+5 in wild-type testis. White lines indicate early spermatocytes. Scale bar: 100 µm in H. (I,J) RT-PCR on CycB-eYFP, CycB-eYFP in rbp4 and CycB-eYFP-mut9+5 reporters. (I) Amplifying eYFP (313-bp expected product) and (control) GAPDH2 (100 bp) to assay reporter transcript levels. (J) Using an eYFP forward primer and cycB 3′ UTR reverse primers 2 and 3. Predicted products: 157 bp from short and long form; 260 bp from long form only – not detected. (K) Anti-GFP western blot of a biotin RNA pull-down from Rbp4-eYFP or Ubi-GFP testis extract. Wild-type and mutant biotin probes as indicated. (L) Quantification via ImageJ of three independent biotin RNA pull-downs from Rbp4-eYFP. The mean of the 10% input bands was set to 1; a value of 2 for the wild-type probe indicates that that probe pulled down ∼20% of the Rbp4-eYFP input. Error bars indicate s.e.m.
Mouse Anti Cycb, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti cycb/product/Developmental Studies Hybridoma Bank
Average 94 stars, based on 1 article reviews
mouse anti cycb - by Bioz Stars, 2026-03
94/100 stars
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a11122  (Thermo Fisher)
86
Thermo Fisher a11122
Sequences in the <t>cycB</t> 3′ UTR are required for translational repression. (A) 3′ RACE PCR on the cycB transcript from RNA collected from wild-type testis, wild-type ovary, bam mutant testis (spermatogonia accumulate, and spermatocytes and spermatids are absent). Forward primer for 3′ RACE was nearly identical to primer #1 in C, just 4 bases longer. (B) RT-PCR from RNA collected from wild-type testis, wild-type ovary, bam mutant testis and rbp4 mutant testis, using primers #1-3, shown in C. Predicted products: 124 bp from short and long form; 227 bp from long form only. (C) Schematic of the cycB 3′ UTR as detected in ovary and spermatogonia versus in spermatocytes. NRE, Nanos response element. (D) A conserved proportion of the short cycB 3′ UTR, with mut9+5 variation created by site-directed mutagenesis (mutated nucleotides shown in lowercase). (E) Schematic of CycB-eYFP in vivo reporter. <t>(F-J)</t> <t>Anti-GFP</t> immunofluorescence on: (F) CycB-eYFP in wild-type testis, (G) CycB-eYFP in rbp4 mutant testis and (H) CycB-eYFP-mut9+5 in wild-type testis. White lines indicate early spermatocytes. Scale bar: 100 µm in H. (I,J) RT-PCR on CycB-eYFP, CycB-eYFP in rbp4 and CycB-eYFP-mut9+5 reporters. (I) Amplifying eYFP (313-bp expected product) and (control) GAPDH2 (100 bp) to assay reporter transcript levels. (J) Using an eYFP forward primer and cycB 3′ UTR reverse primers 2 and 3. Predicted products: 157 bp from short and long form; 260 bp from long form only – not detected. (K) Anti-GFP western blot of a biotin RNA pull-down from Rbp4-eYFP or Ubi-GFP testis extract. Wild-type and mutant biotin probes as indicated. (L) Quantification via ImageJ of three independent biotin RNA pull-downs from Rbp4-eYFP. The mean of the 10% input bands was set to 1; a value of 2 for the wild-type probe indicates that that probe pulled down ∼20% of the Rbp4-eYFP input. Error bars indicate s.e.m.
A11122, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/a11122/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
a11122 - by Bioz Stars, 2026-03
86/100 stars
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hybridoma product  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank hybridoma product
Sequences in the <t>cycB</t> 3′ UTR are required for translational repression. (A) 3′ RACE PCR on the cycB transcript from RNA collected from wild-type testis, wild-type ovary, bam mutant testis (spermatogonia accumulate, and spermatocytes and spermatids are absent). Forward primer for 3′ RACE was nearly identical to primer #1 in C, just 4 bases longer. (B) RT-PCR from RNA collected from wild-type testis, wild-type ovary, bam mutant testis and rbp4 mutant testis, using primers #1-3, shown in C. Predicted products: 124 bp from short and long form; 227 bp from long form only. (C) Schematic of the cycB 3′ UTR as detected in ovary and spermatogonia versus in spermatocytes. NRE, Nanos response element. (D) A conserved proportion of the short cycB 3′ UTR, with mut9+5 variation created by site-directed mutagenesis (mutated nucleotides shown in lowercase). (E) Schematic of CycB-eYFP in vivo reporter. <t>(F-J)</t> <t>Anti-GFP</t> immunofluorescence on: (F) CycB-eYFP in wild-type testis, (G) CycB-eYFP in rbp4 mutant testis and (H) CycB-eYFP-mut9+5 in wild-type testis. White lines indicate early spermatocytes. Scale bar: 100 µm in H. (I,J) RT-PCR on CycB-eYFP, CycB-eYFP in rbp4 and CycB-eYFP-mut9+5 reporters. (I) Amplifying eYFP (313-bp expected product) and (control) GAPDH2 (100 bp) to assay reporter transcript levels. (J) Using an eYFP forward primer and cycB 3′ UTR reverse primers 2 and 3. Predicted products: 157 bp from short and long form; 260 bp from long form only – not detected. (K) Anti-GFP western blot of a biotin RNA pull-down from Rbp4-eYFP or Ubi-GFP testis extract. Wild-type and mutant biotin probes as indicated. (L) Quantification via ImageJ of three independent biotin RNA pull-downs from Rbp4-eYFP. The mean of the 10% input bands was set to 1; a value of 2 for the wild-type probe indicates that that probe pulled down ∼20% of the Rbp4-eYFP input. Error bars indicate s.e.m.
Hybridoma Product, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hybridoma product/product/Developmental Studies Hybridoma Bank
Average 94 stars, based on 1 article reviews
hybridoma product - by Bioz Stars, 2026-03
94/100 stars
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mouse anti cut  (Developmental Studies Hybridoma Bank)
96
Developmental Studies Hybridoma Bank mouse anti cut
Sequences in the <t>cycB</t> 3′ UTR are required for translational repression. (A) 3′ RACE PCR on the cycB transcript from RNA collected from wild-type testis, wild-type ovary, bam mutant testis (spermatogonia accumulate, and spermatocytes and spermatids are absent). Forward primer for 3′ RACE was nearly identical to primer #1 in C, just 4 bases longer. (B) RT-PCR from RNA collected from wild-type testis, wild-type ovary, bam mutant testis and rbp4 mutant testis, using primers #1-3, shown in C. Predicted products: 124 bp from short and long form; 227 bp from long form only. (C) Schematic of the cycB 3′ UTR as detected in ovary and spermatogonia versus in spermatocytes. NRE, Nanos response element. (D) A conserved proportion of the short cycB 3′ UTR, with mut9+5 variation created by site-directed mutagenesis (mutated nucleotides shown in lowercase). (E) Schematic of CycB-eYFP in vivo reporter. <t>(F-J)</t> <t>Anti-GFP</t> immunofluorescence on: (F) CycB-eYFP in wild-type testis, (G) CycB-eYFP in rbp4 mutant testis and (H) CycB-eYFP-mut9+5 in wild-type testis. White lines indicate early spermatocytes. Scale bar: 100 µm in H. (I,J) RT-PCR on CycB-eYFP, CycB-eYFP in rbp4 and CycB-eYFP-mut9+5 reporters. (I) Amplifying eYFP (313-bp expected product) and (control) GAPDH2 (100 bp) to assay reporter transcript levels. (J) Using an eYFP forward primer and cycB 3′ UTR reverse primers 2 and 3. Predicted products: 157 bp from short and long form; 260 bp from long form only – not detected. (K) Anti-GFP western blot of a biotin RNA pull-down from Rbp4-eYFP or Ubi-GFP testis extract. Wild-type and mutant biotin probes as indicated. (L) Quantification via ImageJ of three independent biotin RNA pull-downs from Rbp4-eYFP. The mean of the 10% input bands was set to 1; a value of 2 for the wild-type probe indicates that that probe pulled down ∼20% of the Rbp4-eYFP input. Error bars indicate s.e.m.
Mouse Anti Cut, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
mouse anti cut - by Bioz Stars, 2026-03
96/100 stars
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mouse fk2  (Stressgen Biotechnologies)
90
Stressgen Biotechnologies mouse fk2
Sequences in the <t>cycB</t> 3′ UTR are required for translational repression. (A) 3′ RACE PCR on the cycB transcript from RNA collected from wild-type testis, wild-type ovary, bam mutant testis (spermatogonia accumulate, and spermatocytes and spermatids are absent). Forward primer for 3′ RACE was nearly identical to primer #1 in C, just 4 bases longer. (B) RT-PCR from RNA collected from wild-type testis, wild-type ovary, bam mutant testis and rbp4 mutant testis, using primers #1-3, shown in C. Predicted products: 124 bp from short and long form; 227 bp from long form only. (C) Schematic of the cycB 3′ UTR as detected in ovary and spermatogonia versus in spermatocytes. NRE, Nanos response element. (D) A conserved proportion of the short cycB 3′ UTR, with mut9+5 variation created by site-directed mutagenesis (mutated nucleotides shown in lowercase). (E) Schematic of CycB-eYFP in vivo reporter. <t>(F-J)</t> <t>Anti-GFP</t> immunofluorescence on: (F) CycB-eYFP in wild-type testis, (G) CycB-eYFP in rbp4 mutant testis and (H) CycB-eYFP-mut9+5 in wild-type testis. White lines indicate early spermatocytes. Scale bar: 100 µm in H. (I,J) RT-PCR on CycB-eYFP, CycB-eYFP in rbp4 and CycB-eYFP-mut9+5 reporters. (I) Amplifying eYFP (313-bp expected product) and (control) GAPDH2 (100 bp) to assay reporter transcript levels. (J) Using an eYFP forward primer and cycB 3′ UTR reverse primers 2 and 3. Predicted products: 157 bp from short and long form; 260 bp from long form only – not detected. (K) Anti-GFP western blot of a biotin RNA pull-down from Rbp4-eYFP or Ubi-GFP testis extract. Wild-type and mutant biotin probes as indicated. (L) Quantification via ImageJ of three independent biotin RNA pull-downs from Rbp4-eYFP. The mean of the 10% input bands was set to 1; a value of 2 for the wild-type probe indicates that that probe pulled down ∼20% of the Rbp4-eYFP input. Error bars indicate s.e.m.
Mouse Fk2, supplied by Stressgen Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mouse fk2 - by Bioz Stars, 2026-03
90/100 stars
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rabbit anti-ß-galactosidase  (Cappel Laboratories)
90
Cappel Laboratories rabbit anti-ß-galactosidase
Sequences in the <t>cycB</t> 3′ UTR are required for translational repression. (A) 3′ RACE PCR on the cycB transcript from RNA collected from wild-type testis, wild-type ovary, bam mutant testis (spermatogonia accumulate, and spermatocytes and spermatids are absent). Forward primer for 3′ RACE was nearly identical to primer #1 in C, just 4 bases longer. (B) RT-PCR from RNA collected from wild-type testis, wild-type ovary, bam mutant testis and rbp4 mutant testis, using primers #1-3, shown in C. Predicted products: 124 bp from short and long form; 227 bp from long form only. (C) Schematic of the cycB 3′ UTR as detected in ovary and spermatogonia versus in spermatocytes. NRE, Nanos response element. (D) A conserved proportion of the short cycB 3′ UTR, with mut9+5 variation created by site-directed mutagenesis (mutated nucleotides shown in lowercase). (E) Schematic of CycB-eYFP in vivo reporter. <t>(F-J)</t> <t>Anti-GFP</t> immunofluorescence on: (F) CycB-eYFP in wild-type testis, (G) CycB-eYFP in rbp4 mutant testis and (H) CycB-eYFP-mut9+5 in wild-type testis. White lines indicate early spermatocytes. Scale bar: 100 µm in H. (I,J) RT-PCR on CycB-eYFP, CycB-eYFP in rbp4 and CycB-eYFP-mut9+5 reporters. (I) Amplifying eYFP (313-bp expected product) and (control) GAPDH2 (100 bp) to assay reporter transcript levels. (J) Using an eYFP forward primer and cycB 3′ UTR reverse primers 2 and 3. Predicted products: 157 bp from short and long form; 260 bp from long form only – not detected. (K) Anti-GFP western blot of a biotin RNA pull-down from Rbp4-eYFP or Ubi-GFP testis extract. Wild-type and mutant biotin probes as indicated. (L) Quantification via ImageJ of three independent biotin RNA pull-downs from Rbp4-eYFP. The mean of the 10% input bands was set to 1; a value of 2 for the wild-type probe indicates that that probe pulled down ∼20% of the Rbp4-eYFP input. Error bars indicate s.e.m.
Rabbit Anti ß Galactosidase, supplied by Cappel Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-ß-galactosidase/product/Cappel Laboratories
Average 90 stars, based on 1 article reviews
rabbit anti-ß-galactosidase - by Bioz Stars, 2026-03
90/100 stars
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rabbit anti vasa  (Developmental Studies Hybridoma Bank)
97
Developmental Studies Hybridoma Bank rabbit anti vasa
Sequences in the <t>cycB</t> 3′ UTR are required for translational repression. (A) 3′ RACE PCR on the cycB transcript from RNA collected from wild-type testis, wild-type ovary, bam mutant testis (spermatogonia accumulate, and spermatocytes and spermatids are absent). Forward primer for 3′ RACE was nearly identical to primer #1 in C, just 4 bases longer. (B) RT-PCR from RNA collected from wild-type testis, wild-type ovary, bam mutant testis and rbp4 mutant testis, using primers #1-3, shown in C. Predicted products: 124 bp from short and long form; 227 bp from long form only. (C) Schematic of the cycB 3′ UTR as detected in ovary and spermatogonia versus in spermatocytes. NRE, Nanos response element. (D) A conserved proportion of the short cycB 3′ UTR, with mut9+5 variation created by site-directed mutagenesis (mutated nucleotides shown in lowercase). (E) Schematic of CycB-eYFP in vivo reporter. <t>(F-J)</t> <t>Anti-GFP</t> immunofluorescence on: (F) CycB-eYFP in wild-type testis, (G) CycB-eYFP in rbp4 mutant testis and (H) CycB-eYFP-mut9+5 in wild-type testis. White lines indicate early spermatocytes. Scale bar: 100 µm in H. (I,J) RT-PCR on CycB-eYFP, CycB-eYFP in rbp4 and CycB-eYFP-mut9+5 reporters. (I) Amplifying eYFP (313-bp expected product) and (control) GAPDH2 (100 bp) to assay reporter transcript levels. (J) Using an eYFP forward primer and cycB 3′ UTR reverse primers 2 and 3. Predicted products: 157 bp from short and long form; 260 bp from long form only – not detected. (K) Anti-GFP western blot of a biotin RNA pull-down from Rbp4-eYFP or Ubi-GFP testis extract. Wild-type and mutant biotin probes as indicated. (L) Quantification via ImageJ of three independent biotin RNA pull-downs from Rbp4-eYFP. The mean of the 10% input bands was set to 1; a value of 2 for the wild-type probe indicates that that probe pulled down ∼20% of the Rbp4-eYFP input. Error bars indicate s.e.m.
Rabbit Anti Vasa, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti vasa/product/Developmental Studies Hybridoma Bank
Average 97 stars, based on 1 article reviews
rabbit anti vasa - by Bioz Stars, 2026-03
97/100 stars
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mouse anti actin  (Developmental Studies Hybridoma Bank)
97
Developmental Studies Hybridoma Bank mouse anti actin
Sequences in the <t>cycB</t> 3′ UTR are required for translational repression. (A) 3′ RACE PCR on the cycB transcript from RNA collected from wild-type testis, wild-type ovary, bam mutant testis (spermatogonia accumulate, and spermatocytes and spermatids are absent). Forward primer for 3′ RACE was nearly identical to primer #1 in C, just 4 bases longer. (B) RT-PCR from RNA collected from wild-type testis, wild-type ovary, bam mutant testis and rbp4 mutant testis, using primers #1-3, shown in C. Predicted products: 124 bp from short and long form; 227 bp from long form only. (C) Schematic of the cycB 3′ UTR as detected in ovary and spermatogonia versus in spermatocytes. NRE, Nanos response element. (D) A conserved proportion of the short cycB 3′ UTR, with mut9+5 variation created by site-directed mutagenesis (mutated nucleotides shown in lowercase). (E) Schematic of CycB-eYFP in vivo reporter. <t>(F-J)</t> <t>Anti-GFP</t> immunofluorescence on: (F) CycB-eYFP in wild-type testis, (G) CycB-eYFP in rbp4 mutant testis and (H) CycB-eYFP-mut9+5 in wild-type testis. White lines indicate early spermatocytes. Scale bar: 100 µm in H. (I,J) RT-PCR on CycB-eYFP, CycB-eYFP in rbp4 and CycB-eYFP-mut9+5 reporters. (I) Amplifying eYFP (313-bp expected product) and (control) GAPDH2 (100 bp) to assay reporter transcript levels. (J) Using an eYFP forward primer and cycB 3′ UTR reverse primers 2 and 3. Predicted products: 157 bp from short and long form; 260 bp from long form only – not detected. (K) Anti-GFP western blot of a biotin RNA pull-down from Rbp4-eYFP or Ubi-GFP testis extract. Wild-type and mutant biotin probes as indicated. (L) Quantification via ImageJ of three independent biotin RNA pull-downs from Rbp4-eYFP. The mean of the 10% input bands was set to 1; a value of 2 for the wild-type probe indicates that that probe pulled down ∼20% of the Rbp4-eYFP input. Error bars indicate s.e.m.
Mouse Anti Actin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti actin/product/Developmental Studies Hybridoma Bank
Average 97 stars, based on 1 article reviews
mouse anti actin - by Bioz Stars, 2026-03
97/100 stars
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rabbit anti–β-galactosidase  (Millipore)
90
Millipore rabbit anti–β-galactosidase
Sequences in the <t>cycB</t> 3′ UTR are required for translational repression. (A) 3′ RACE PCR on the cycB transcript from RNA collected from wild-type testis, wild-type ovary, bam mutant testis (spermatogonia accumulate, and spermatocytes and spermatids are absent). Forward primer for 3′ RACE was nearly identical to primer #1 in C, just 4 bases longer. (B) RT-PCR from RNA collected from wild-type testis, wild-type ovary, bam mutant testis and rbp4 mutant testis, using primers #1-3, shown in C. Predicted products: 124 bp from short and long form; 227 bp from long form only. (C) Schematic of the cycB 3′ UTR as detected in ovary and spermatogonia versus in spermatocytes. NRE, Nanos response element. (D) A conserved proportion of the short cycB 3′ UTR, with mut9+5 variation created by site-directed mutagenesis (mutated nucleotides shown in lowercase). (E) Schematic of CycB-eYFP in vivo reporter. <t>(F-J)</t> <t>Anti-GFP</t> immunofluorescence on: (F) CycB-eYFP in wild-type testis, (G) CycB-eYFP in rbp4 mutant testis and (H) CycB-eYFP-mut9+5 in wild-type testis. White lines indicate early spermatocytes. Scale bar: 100 µm in H. (I,J) RT-PCR on CycB-eYFP, CycB-eYFP in rbp4 and CycB-eYFP-mut9+5 reporters. (I) Amplifying eYFP (313-bp expected product) and (control) GAPDH2 (100 bp) to assay reporter transcript levels. (J) Using an eYFP forward primer and cycB 3′ UTR reverse primers 2 and 3. Predicted products: 157 bp from short and long form; 260 bp from long form only – not detected. (K) Anti-GFP western blot of a biotin RNA pull-down from Rbp4-eYFP or Ubi-GFP testis extract. Wild-type and mutant biotin probes as indicated. (L) Quantification via ImageJ of three independent biotin RNA pull-downs from Rbp4-eYFP. The mean of the 10% input bands was set to 1; a value of 2 for the wild-type probe indicates that that probe pulled down ∼20% of the Rbp4-eYFP input. Error bars indicate s.e.m.
Rabbit Anti–β Galactosidase, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
rabbit anti–β-galactosidase - by Bioz Stars, 2026-03
90/100 stars
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Sequences in the cycB 3′ UTR are required for translational repression. (A) 3′ RACE PCR on the cycB transcript from RNA collected from wild-type testis, wild-type ovary, bam mutant testis (spermatogonia accumulate, and spermatocytes and spermatids are absent). Forward primer for 3′ RACE was nearly identical to primer #1 in C, just 4 bases longer. (B) RT-PCR from RNA collected from wild-type testis, wild-type ovary, bam mutant testis and rbp4 mutant testis, using primers #1-3, shown in C. Predicted products: 124 bp from short and long form; 227 bp from long form only. (C) Schematic of the cycB 3′ UTR as detected in ovary and spermatogonia versus in spermatocytes. NRE, Nanos response element. (D) A conserved proportion of the short cycB 3′ UTR, with mut9+5 variation created by site-directed mutagenesis (mutated nucleotides shown in lowercase). (E) Schematic of CycB-eYFP in vivo reporter. (F-J) Anti-GFP immunofluorescence on: (F) CycB-eYFP in wild-type testis, (G) CycB-eYFP in rbp4 mutant testis and (H) CycB-eYFP-mut9+5 in wild-type testis. White lines indicate early spermatocytes. Scale bar: 100 µm in H. (I,J) RT-PCR on CycB-eYFP, CycB-eYFP in rbp4 and CycB-eYFP-mut9+5 reporters. (I) Amplifying eYFP (313-bp expected product) and (control) GAPDH2 (100 bp) to assay reporter transcript levels. (J) Using an eYFP forward primer and cycB 3′ UTR reverse primers 2 and 3. Predicted products: 157 bp from short and long form; 260 bp from long form only – not detected. (K) Anti-GFP western blot of a biotin RNA pull-down from Rbp4-eYFP or Ubi-GFP testis extract. Wild-type and mutant biotin probes as indicated. (L) Quantification via ImageJ of three independent biotin RNA pull-downs from Rbp4-eYFP. The mean of the 10% input bands was set to 1; a value of 2 for the wild-type probe indicates that that probe pulled down ∼20% of the Rbp4-eYFP input. Error bars indicate s.e.m.

Journal: Development (Cambridge, England)

Article Title: Cell type-specific translational repression of Cyclin B during meiosis in males

doi: 10.1242/dev.122341

Figure Lengend Snippet: Sequences in the cycB 3′ UTR are required for translational repression. (A) 3′ RACE PCR on the cycB transcript from RNA collected from wild-type testis, wild-type ovary, bam mutant testis (spermatogonia accumulate, and spermatocytes and spermatids are absent). Forward primer for 3′ RACE was nearly identical to primer #1 in C, just 4 bases longer. (B) RT-PCR from RNA collected from wild-type testis, wild-type ovary, bam mutant testis and rbp4 mutant testis, using primers #1-3, shown in C. Predicted products: 124 bp from short and long form; 227 bp from long form only. (C) Schematic of the cycB 3′ UTR as detected in ovary and spermatogonia versus in spermatocytes. NRE, Nanos response element. (D) A conserved proportion of the short cycB 3′ UTR, with mut9+5 variation created by site-directed mutagenesis (mutated nucleotides shown in lowercase). (E) Schematic of CycB-eYFP in vivo reporter. (F-J) Anti-GFP immunofluorescence on: (F) CycB-eYFP in wild-type testis, (G) CycB-eYFP in rbp4 mutant testis and (H) CycB-eYFP-mut9+5 in wild-type testis. White lines indicate early spermatocytes. Scale bar: 100 µm in H. (I,J) RT-PCR on CycB-eYFP, CycB-eYFP in rbp4 and CycB-eYFP-mut9+5 reporters. (I) Amplifying eYFP (313-bp expected product) and (control) GAPDH2 (100 bp) to assay reporter transcript levels. (J) Using an eYFP forward primer and cycB 3′ UTR reverse primers 2 and 3. Predicted products: 157 bp from short and long form; 260 bp from long form only – not detected. (K) Anti-GFP western blot of a biotin RNA pull-down from Rbp4-eYFP or Ubi-GFP testis extract. Wild-type and mutant biotin probes as indicated. (L) Quantification via ImageJ of three independent biotin RNA pull-downs from Rbp4-eYFP. The mean of the 10% input bands was set to 1; a value of 2 for the wild-type probe indicates that that probe pulled down ∼20% of the Rbp4-eYFP input. Error bars indicate s.e.m.

Article Snippet: Antibody sources and dilutions: anti-CycB (1:30; F2F4, mouse, BD Biosciences), anti-HA (1:1000; 16B12, mouse, Covance), anti-GFP (1:3000; # {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966","term_text":"A11122"}} A11122 , rabbit, Invitrogen/Life Technologies), anti-PH3Thr3 (1:200; #07-424, rabbit, Millipore).

Techniques: Mutagenesis, Reverse Transcription Polymerase Chain Reaction, In Vivo, Immunofluorescence, Western Blot

A 31-amino acid domain in Rbp4 is required for binding to Fest. (A) Diagram of Rbp4 protein structure and truncated Rbp4 proteins tested in S2 cells for binding to Fest. RRM, RNA recognition motif. (B) Anti-HA, anti-Myc western blot of anti-Myc immunoprecipitations (top panels) and 10% input (bottom panels) from S2 cells transfected with HA-Fest alone or HA-Fest with various Myc-Rbp4 truncations, as indicated. (C) Alignment of Rbp4 residues 245-275 with Rbp4 homologs in other species. (D,E) Anti-GFP immunostaining of Rbp4-eYFP/+ testis (D) and Rbp4-eYFP/+; fest testis (E). Scale bar: 100 µm in E for D and E. (F) Biotin RNA pull-down from Rbp4-eYFP/+, fest testis, using the wild-type 130 nt cycB 3′ UTR probe.

Journal: Development (Cambridge, England)

Article Title: Cell type-specific translational repression of Cyclin B during meiosis in males

doi: 10.1242/dev.122341

Figure Lengend Snippet: A 31-amino acid domain in Rbp4 is required for binding to Fest. (A) Diagram of Rbp4 protein structure and truncated Rbp4 proteins tested in S2 cells for binding to Fest. RRM, RNA recognition motif. (B) Anti-HA, anti-Myc western blot of anti-Myc immunoprecipitations (top panels) and 10% input (bottom panels) from S2 cells transfected with HA-Fest alone or HA-Fest with various Myc-Rbp4 truncations, as indicated. (C) Alignment of Rbp4 residues 245-275 with Rbp4 homologs in other species. (D,E) Anti-GFP immunostaining of Rbp4-eYFP/+ testis (D) and Rbp4-eYFP/+; fest testis (E). Scale bar: 100 µm in E for D and E. (F) Biotin RNA pull-down from Rbp4-eYFP/+, fest testis, using the wild-type 130 nt cycB 3′ UTR probe.

Article Snippet: Antibody sources and dilutions: anti-CycB (1:30; F2F4, mouse, BD Biosciences), anti-HA (1:1000; 16B12, mouse, Covance), anti-GFP (1:3000; # {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966","term_text":"A11122"}} A11122 , rabbit, Invitrogen/Life Technologies), anti-PH3Thr3 (1:200; #07-424, rabbit, Millipore).

Techniques: Binding Assay, Western Blot, Transfection, Immunostaining